Why you should not use FPKM’s
Why you should not use FPKM’s …
FPKM’s are often requested as output from the Bioinformatic Core’s RNAseq pipeline. There are several issues/problems with using them. First they should not be used at all. The originator of FPKM’s himself feels they are not an appropriate method to normalize RNAseq data. Please see the following from Dr. Pachter explaining the problems and what should be used instead:
