Core develops new pipeline for spatial single cell proteomic analysis
Core staff members Caitlin Jones and Mono Pirun and core alumni Zheng Zeng have been working on a new analysis project to analyze spatial single cell multiplex proteomic data in collaboration with the Ingo Mellinghoff and Travis Hollmann labs. This…
Updates from the Bioinformatics Core: Virtual Clinics, Resources, and More
The bioinformatics core continues to be available to assist the research community. We want to let you know how to best contact us and share with you some resources: Virtual Clinics Members of the bioinformatics core are available to answer…
Suggested Reading: 2020-03-22
For researchers interested in learning best-practices for bioinformatics/computational biology, the following blog post has links to a number of interesting papers. Ten great papers for biologists starting out in computational biology https://widdowquinn.github.io/ten_great_papers/ From: Leighton Pritchard (@widdowquinn) Are you a wet…
Why you should not use FPKM’s
Why you should not use FPKM's ... FPKM's are often requested as output from the Bioinformatic Core's RNAseq pipeline. There are several issues/problems with using them. First they should not be used at all. The originator of FPKM's himself feels…
How many biological replicates are needed in an RNA-seq experiment?
SUMMARY: An RNA-seq experiment with 48 biological replicates in each of two conditions was performed to answer these questions and provide guidelines for experimental design. With three biological replicates, eight of the 11 tools evaluated found only 20%–40% of the significantly…
